This microsatellite dataset was constructed using eight microsatellite loci with 270 individual samples, representing wild population of Euonymus fortunei in Ohio, Kentucky, Kansas, and Minnesota. Also represented are multiple individuals from several Euonymus cultivars and also wild E. alatus (burning bush) from Ohio. This database is published as Elam RJ and Culley TM (2023) Genetic Analysis of Invasive Spread of Euonymus fortunei (Wintercreeper), a Popular Ornamental Groundcover. Invasive Plant Science and Management.