The Auditory Brainstem Response (ABR) to gated tone burst of five frequencies (0.125, 0.5, 4, 8, 10 kHz) as well as broadband click stimuli were recorded in two cheetahs, one male and one female. We report, for the first time, hearing estimates from two cheetahs. Provided are the raw data files in PDF format and RPT format which is the file format used by the Intelligent Hearing System Universal Smart Box for analysis and to mark waves in order to obtain amplitude and latency of waves. Data were obtained for the female cheetah on February 1, 2024 and for the male cheetah on March 26, 2024 from the Cincinnati Zoo and Botanical Gardens.