{"response":{"docs":[{"system_create_dtsi":"2017-10-27T02:51:52Z","system_modified_dtsi":"2018-08-01T18:40:09Z","has_model_ssim":["Article"],"id":"bc387081x","accessControl_ssim":["add9072d-1ff4-4d50-a075-c58b872a6fc5"],"hasRelatedMediaFragment_ssim":["bc3870826"],"hasRelatedImage_ssim":["bc3870826"],"depositor_ssim":["greiskd@ucmail.uc.edu"],"depositor_tesim":["greiskd@ucmail.uc.edu"],"title_tesim":["Purification and Characterization an Extracellular Phosphoglycan from Leishmania donovani"],"date_uploaded_dtsi":"2017-03-01T00:00:00Z","date_modified_dtsi":"2017-04-07T00:00:00Z","isPartOf_ssim":["admin_set/default"],"proxy_depositor_ssim":["dungavjk@mail.uc.edu"],"journal_title_tesim":["Journal of Biological Chemistry"],"college_tesim":["Medicine"],"department_tesim":["Cancer \u0026 Cell Biology"],"note_tesim":["This work was part of a pilot \"mediated-deposit model\" where library staff found potential works, later submitted for faculty review"],"creator_tesim":["Turco, Salvatore J.","Thomas, Jerry R.","Homans, Steven W.","Ferguson, Michael A.","McConville, Malcolm J.","Greis, Kenneth D."],"subject_tesim":["Biological Chemistry","Biology"],"language_tesim":["English"],"description_tesim":["An extracellular phosphoglycan (exPG), present in the culturem edium of the promastigote form L oefi shmania donovani, was purified and structurally characterized. The purification scheme included ethanol precipitation of the culture medium, anion exchange chromatography, hydrophobic chromatography on phenyl-Sepharose, and preparative polyacrylamgeild e electrophoresis. Structural analysis by ‘H-’H NMR, methylation linkage analysis, and glycosidase digestion revealed that the exPG consisted of thfoel lowing structure: (CAP)+[P04-6Galp@1-4Manpal]lo-11-POr6GalpB1-4Man. The capw as found to be ones eovf eral small, neutral oligosaccharides, the most abundant of which was the trisaccharide Galp@l-4(Manpal-2)Man. The results indicated structural analogy to the cellular-derived lipophosphoglycan (LPG) from L. donovani. The important exceptions are a lacko f the lipid anchor, the entire phosphosaccharide core, and several of the repeating disaccharide units. Although the function of exPGis presently unknowni,t may play a protective role for the promastigote in the insect vector or during infection of a mammalian host "],"license_tesim":["http://rightsstatements.org/vocab/InC/1.0/"],"date_created_tesim":["1992-04"],"source_tesim":["Journal of Biological Chemistry"],"thumbnail_path_ss":"/downloads/bc3870826?file=thumbnail","suppressed_bsi":false,"actionable_workflow_roles_ssim":["admin_set/default-default-depositing"],"workflow_state_name_ssim":["deposited"],"member_ids_ssim":["bc3870826"],"file_set_ids_ssim":["bc3870826"],"visibility_ssi":"open","admin_set_tesim":["Default Admin Set"],"sort_title_ssi":"PURIFICATION AND CHARACTERIZATION AN EXTRACELLULAR PHOSPHOGLYCAN FROM LEISHMANIA DONOVANI","human_readable_type_tesim":["Article"],"read_access_group_ssim":["public"],"read_access_person_ssim":["konecnmc@ucmail.uc.edu"],"edit_access_person_ssim":["greiskd@ucmail.uc.edu"],"nesting_collection__pathnames_ssim":["bc387081x"],"nesting_collection__deepest_nested_depth_isi":1,"_version_":1697096093120069632,"timestamp":"2021-04-15T08:55:56.482Z","score":0.00049999997}],"facets":[{"name":"human_readable_type_sim","items":[{"value":"Article","hits":1,"label":"Article"}],"label":"Human Readable Type Sim"},{"name":"creator_sim","items":[{"value":"Ferguson, Michael A.","hits":1,"label":"Ferguson, Michael A."},{"value":"Greis, Kenneth D.","hits":1,"label":"Greis, Kenneth D."},{"value":"Homans, Steven W.","hits":1,"label":"Homans, Steven W."},{"value":"McConville, Malcolm J.","hits":1,"label":"McConville, Malcolm J."},{"value":"Thomas, Jerry R.","hits":1,"label":"Thomas, Jerry R."},{"value":"Turco, Salvatore J.","hits":1,"label":"Turco, Salvatore J."}],"label":"Creator Sim"},{"name":"subject_sim","items":[{"value":"Biological Chemistry","hits":1,"label":"Biological Chemistry"},{"value":"Biology","hits":1,"label":"Biology"}],"label":"Subject Sim"},{"name":"college_sim","items":[{"value":"Medicine","hits":1,"label":"Medicine"}],"label":"College Sim"},{"name":"department_sim","items":[{"value":"Cancer \u0026 Cell Biology","hits":1,"label":"Cancer \u0026 Cell Biology"}],"label":"Department Sim"},{"name":"language_sim","items":[{"value":"English","hits":1,"label":"English"}],"label":"Language Sim"},{"name":"publisher_sim","items":[],"label":"Publisher Sim"},{"name":"date_created_sim","items":[{"value":"1992-04","hits":1,"label":"1992-04"}],"label":"Date Created Sim"},{"name":"member_of_collection_ids_ssim","items":[],"label":"Member Of Collection Ids Ssim"},{"name":"generic_type_sim","items":[{"value":"Work","hits":1,"label":"Work"}],"label":"Generic Type Sim"}],"pages":{"current_page":1,"next_page":null,"prev_page":null,"total_pages":1,"limit_value":10,"offset_value":0,"total_count":1,"first_page?":true,"last_page?":true}}}