{"response":{"docs":[{"system_create_dtsi":"2017-10-27T02:52:36Z","system_modified_dtsi":"2018-08-01T18:40:13Z","has_model_ssim":["Article"],"id":"bc3871016","accessControl_ssim":["f3523d20-8200-4e78-aefa-71ac422195f5"],"hasRelatedMediaFragment_ssim":["bc387102g"],"hasRelatedImage_ssim":["bc387102g"],"depositor_ssim":["greiskd@ucmail.uc.edu"],"depositor_tesim":["greiskd@ucmail.uc.edu"],"title_tesim":["Heparin-Binding Motifs and Biofilm Formation by Candida albicans"],"date_uploaded_dtsi":"2017-03-03T00:00:00Z","date_modified_dtsi":"2017-04-07T00:00:00Z","isPartOf_ssim":["admin_set/default"],"proxy_depositor_ssim":["dungavjk@mail.uc.edu"],"doi_tesim":["10.1093/infdis/jit391"],"journal_title_tesim":["The Journal of Infectious Diseases"],"college_tesim":["Medicine"],"department_tesim":["Cancer \u0026 Cell Biology"],"note_tesim":["This work was part of a pilot \"mediated-deposit model\" where library staff found potential works, later submitted for faculty review"],"creator_tesim":["Andes, David R.","Lu, Jason Long","Tan, Queenie K., G.","Hostetter, Margaret K.","Greis, Kenneth D.","Zhang, Minlu","Orsborn, Kris I.","Porollo, Alexey","Green, Julianne V."],"subject_tesim":["Infectious Diseases","Cancer Biology"],"language_tesim":["English"],"description_tesim":["Candida albicans is a leading pathogen in infections of central venous catheters, which are frequently infused with heparin. Binding of C. albicans to medically relevant concentrations of soluble and plate-bound heparin was demonstrable by confocal microscopy and enzyme-linked immunosorbent assay (ELISA). A sequencebased search identified 34 C. albicans surface proteins containing ≥1 match to linear heparin-binding motifs. The virulence factor Int1 contained the most putative heparin-binding motifs (n = 5); peptides encompassing 2 of 5 motifs bound to heparin-Sepharose. Alanine substitution of lysine residues K805/K806 in 804QKKHQIHK811 (motif 1 of Int1) markedly attenuated biofilm formation in central venous catheters in rats, whereas alanine substitution of K1595/R1596 in 1593FKKRFFKL1600 (motif 4 of Int1) did not impair biofilm formation. Affinity-purified immunoglobulin G (IgG) recognizing motif 1 abolished biofilm formation in central venous catheters; preimmune IgG had no effect. After heparin treatment of C. albicans, soluble peptides from multiple C. albicans surface proteins were detected, such as Eno1, Pgk1, Tdh3, and Ssa1/2 but not Int1, suggesting that heparin changes candidal surface structures and may modify some antigens critical for immune recognition. These studies define a new mechanism of biofilm formation for C. albicans and a novel strategy for inhibiting catheter-associated biofilms."],"license_tesim":["http://rightsstatements.org/vocab/InC/1.0/"],"date_created_tesim":["2013-07"],"source_tesim":["The Journal of Infectious Diseases"],"thumbnail_path_ss":"/downloads/bc387102g?file=thumbnail","suppressed_bsi":false,"actionable_workflow_roles_ssim":["admin_set/default-default-depositing"],"workflow_state_name_ssim":["deposited"],"member_ids_ssim":["bc387102g"],"file_set_ids_ssim":["bc387102g"],"visibility_ssi":"open","admin_set_tesim":["Default Admin Set"],"sort_title_ssi":"HEPARINBINDING MOTIFS AND BIOFILM FORMATION BY CANDIDA ALBICANS","human_readable_type_tesim":["Article"],"read_access_group_ssim":["public"],"read_access_person_ssim":["konecnmc@ucmail.uc.edu"],"edit_access_person_ssim":["greiskd@ucmail.uc.edu"],"nesting_collection__pathnames_ssim":["bc3871016"],"nesting_collection__deepest_nested_depth_isi":1,"_version_":1697120081311432704,"timestamp":"2021-04-15T15:17:13.404Z","score":0.00049999997}],"facets":[{"name":"human_readable_type_sim","items":[{"value":"Article","hits":1,"label":"Article"}],"label":"Human Readable Type Sim"},{"name":"creator_sim","items":[{"value":"Andes, David R.","hits":1,"label":"Andes, David R."},{"value":"Green, Julianne V.","hits":1,"label":"Green, Julianne V."},{"value":"Greis, Kenneth D.","hits":1,"label":"Greis, Kenneth D."},{"value":"Hostetter, Margaret K.","hits":1,"label":"Hostetter, Margaret K."},{"value":"Lu, Jason Long","hits":1,"label":"Lu, Jason Long"},{"value":"Orsborn, Kris I.","hits":1,"label":"Orsborn, Kris I."}],"label":"Creator Sim"},{"name":"subject_sim","items":[{"value":"Cancer Biology","hits":1,"label":"Cancer Biology"},{"value":"Infectious Diseases","hits":1,"label":"Infectious Diseases"}],"label":"Subject Sim"},{"name":"college_sim","items":[{"value":"Medicine","hits":1,"label":"Medicine"}],"label":"College Sim"},{"name":"department_sim","items":[{"value":"Cancer \u0026 Cell Biology","hits":1,"label":"Cancer \u0026 Cell Biology"}],"label":"Department Sim"},{"name":"language_sim","items":[{"value":"English","hits":1,"label":"English"}],"label":"Language Sim"},{"name":"publisher_sim","items":[],"label":"Publisher Sim"},{"name":"date_created_sim","items":[{"value":"2013-07","hits":1,"label":"2013-07"}],"label":"Date Created Sim"},{"name":"member_of_collection_ids_ssim","items":[],"label":"Member Of Collection Ids Ssim"},{"name":"generic_type_sim","items":[{"value":"Work","hits":1,"label":"Work"}],"label":"Generic Type Sim"}],"pages":{"current_page":1,"next_page":null,"prev_page":null,"total_pages":1,"limit_value":10,"offset_value":0,"total_count":1,"first_page?":true,"last_page?":true}}}