{"response":{"docs":[{"system_create_dtsi":"2017-10-27T01:06:11Z","system_modified_dtsi":"2019-06-06T15:08:46Z","has_model_ssim":["Article"],"id":"ff365564x","accessControl_ssim":["6d724178-9527-4911-a40a-73925975088b"],"hasRelatedMediaFragment_ssim":["ff3655656"],"hasRelatedImage_ssim":["ff3655656"],"depositor_ssim":["joffritm@ucmail.uc.edu"],"depositor_tesim":["joffritm@ucmail.uc.edu"],"title_tesim":["Functional Characterization and Localization of Pneumocystis lanosterol synthase"],"date_uploaded_dtsi":"2016-04-14T00:00:00Z","date_modified_dtsi":"2016-04-14T00:00:00Z","isPartOf_ssim":["admin_set/default"],"doi_tesim":["doi:10.7945/C2HS33"],"college_tesim":["Other"],"department_tesim":["HSL Info \u0026 Edu Svcs"],"creator_tesim":["Grant, Tiffany"],"publisher_tesim":["Eukaryotic Cell"],"description_tesim":["Organisms in the genus Pneumocystis are ubiquitous, opportunistic pathogenic fungi capable of causing a\nlethal pneumonia in immunocompromised mammalian hosts. Pneumocystis spp. are unique members of the\nfungal kingdom due to the absence of ergosterol in their cellular membranes. Although these organisms were\nthought to obtain cholesterol by scavenging, transcriptional analyses indicate that Pneumocystis carinii encodes\ngene homologs involved in sterol biosynthesis. To better understand the sterol pathway in these uncultivable\nfungi, yeast deletion strains were used to interrogate the function and localization of P. carinii lanosterol synthase (ERG7). The expression of PcErg7p in an ERG7-null mutant of the yeast Saccharomyces cerevisiae did not alter its growth rate and produced a functional lanosterol synthase, as evidenced by the presence of\nlanosterol detected by gas chromatographic analysis in levels comparable to that produced by the yeast enzyme. Western blotting and fluorescence microscopy revealed that, like the S. cerevisiae Erg7p, the PcErg7p localized to lipid particles in yeast. Using fluorescence microscopy, we show for the first time the presence of apparent lipid particles in P. carinii and the localization of PcErg7p to lipid particles in P. carinii. The detection of lipid particles in P. carinii and their association with PcErg7p therein provide strong evidence that the enzyme serves a similar function in P. carinii. Moreover, the yeast heterologous system should be a useful tool for further analysis of the P. carinii sterol pathway."],"license_tesim":["http://rightsstatements.org/vocab/InC/1.0/"],"thumbnail_path_ss":"/downloads/ff3655656?file=thumbnail","suppressed_bsi":false,"actionable_workflow_roles_ssim":["admin_set/default-default-depositing"],"workflow_state_name_ssim":["deposited"],"member_ids_ssim":["ff3655656"],"file_set_ids_ssim":["ff3655656"],"visibility_ssi":"open","admin_set_tesim":["Default Admin Set"],"sort_title_ssi":"FUNCTIONAL CHARACTERIZATION AND LOCALIZATION OF PNEUMOCYSTIS LANOSTEROL SYNTHASE","human_readable_type_tesim":["Article"],"read_access_group_ssim":["public"],"edit_access_person_ssim":["joffritm@ucmail.uc.edu"],"nesting_collection__pathnames_ssim":["ff365564x"],"nesting_collection__deepest_nested_depth_isi":1,"_version_":1697076654875082752,"timestamp":"2021-04-15T03:46:58.727Z","score":0.00049999997}],"facets":[{"name":"human_readable_type_sim","items":[{"value":"Article","hits":1,"label":"Article"}],"label":"Human Readable Type Sim"},{"name":"creator_sim","items":[{"value":"Grant, Tiffany","hits":1,"label":"Grant, Tiffany"}],"label":"Creator Sim"},{"name":"subject_sim","items":[],"label":"Subject Sim"},{"name":"college_sim","items":[{"value":"Other","hits":1,"label":"Other"}],"label":"College Sim"},{"name":"department_sim","items":[{"value":"HSL Info \u0026 Edu Svcs","hits":1,"label":"HSL Info \u0026 Edu Svcs"}],"label":"Department Sim"},{"name":"language_sim","items":[],"label":"Language Sim"},{"name":"publisher_sim","items":[{"value":"Eukaryotic Cell","hits":1,"label":"Eukaryotic Cell"}],"label":"Publisher Sim"},{"name":"date_created_sim","items":[],"label":"Date Created Sim"},{"name":"member_of_collection_ids_ssim","items":[],"label":"Member Of Collection Ids Ssim"},{"name":"generic_type_sim","items":[{"value":"Work","hits":1,"label":"Work"}],"label":"Generic Type Sim"}],"pages":{"current_page":1,"next_page":null,"prev_page":null,"total_pages":1,"limit_value":10,"offset_value":0,"total_count":1,"first_page?":true,"last_page?":true}}}