Ball milling is used in industry to grind ores, minerals, ceramics, and recently has been implemented to research synthesis of organic compounds. One project is researching nickel catalysis in high speed ball milling for producing substituted cyclooctatetraene compounds. In the process of synthesizing the compounds, Ni, Cr, and Fe residues from the vials and nickel pellets used for the reaction will remain in the product. To analyze for the metals remaining in the product, Inductively Coupled Plasma paired with Mass Spectrometry was used. Initially used as an internal standard, gallium’s mass was found on the spectrometer. It is suspected to be a false positive caused by a FeO+ or FeN+. The product was analyzed using Agilent Technologies 7700 ICP-MS before and after a filtering process. This helped determine how effective the filtering process was removing metals and what concentration remained at the part per billion level (ppb) in the final product. A calibration curve was made with a blank, 1ppb, 5ppb, 10ppb, 25ppb, and 50ppb with Sc as the internal standard. Results showed that chromium is insignificant (<0.0ppb), and iron is approximately 1ppb in concentration. Before the filtration process, nickel is approximately 10ppb, after filtration, nickel’s concentration is insignificant (<0.0ppb).
The purpose of this project was to develop a method to characterize polymers using size exclusion chromatography. Specifically, high performance liquid chromatography coupled with a refractive index detector (RID) was used. Polyethylene glycol (PEG) was used a standard for this method. The mobile phase used was dimethylformamide (DMF). Three different mixtures of PEG polymers with known molecular weights were analyzed to characterize the molecular weight of polyacrylic acid (PAA). After running all polymers, retention times were taken and plotted on the x-axis along with LogMp (molecular weight) on the y-axis. A calibration curve was created and plugged in the retention time of PAA to the line equation. We then, took 10 to the power of that number and calculated a molecular weight of 52292.97 g/mol. This method was shown to be an effective way to characterize polymers using PEG as a standard. Poly (methyl methacrylate), otherwise known as P(MMA), was also tested using the same method. The known weight of the polymer was 60,500 g/mol. After running the polymer, 23,000 g/mol was calculated to be the molecular weight. This molecular weight shows that certain parameters like polarity needs to be considered when running samples on the HPLC.
Poster presenting current research being done into TRNA. The poster shows initial step to isolate TRNA from the digestive enzyme. The long-term goal of analyzing how TRNA react to different stresses over time is mentioned.