Search Constraints

Start Over
Filtering by: College Medicine Remove constraint College: Medicine

Search Results

  • Additive Solution-7 Reduces the Red Blood Cell Cold Storage Lesion

    Type:
    Article
    Description/Abstract:
    Not Available
    Creator/Author:
    Maes, L. A.; Rugg. N; Dumont, L. J.; Cancelas, Jose A.; Zia, M.; Hess, J. R.; Whitley, P. H.; Siegel, A. H.; Szczepiokowski, Z. M., and Herschel, L.
    Submitter:
    Jose Cancelas
    Date Uploaded:
    02/08/2017
    Date Modified:
    04/10/2017
    Date Created:
    2014-10
    License:
    All rights reserved

  • Advanced hair damage model from ultra-violet radiation in the presence of copper

    Type:
    Article
    Description/Abstract:
    OBJECTIVE: Damage to hair from UV exposure has been well reported in the literature and is known to be a highly complex process involving initiation via absorption of UV light followed by formation and propagation of reactive oxygen species (ROS). The objective of this work was to understand these mechanisms, explain the role of copper in accelerating the formation of ROS and identify strategies to reduce the hair damage caused by these reactive species. METHODS: The location of copper in hair was measured by Transmission electron microscopy–(TEM) X-ray energy dispersive spectroscopy (XEDS) and levels measured by ICP-OES. Protein changes were measured as total protein loss via the Lowry assay, and MALDI ToF was used to identify the biomarker protein fragments. TBARS assay was used to measure lipid peroxide formation. Sensory methods and dry combing friction were used to measure hair damage due to copper and UV exposure and to demonstrate the efficacy of N,N’ ethylenediamine disuccinic acid (EDDS) and histidine chelants to reduce this damage. RESULTS: In this work, a biomarker protein fragment formed during UV exposure is identified using mass spectrometry. This fragment originates from the calcium-binding protein S100A3. Also shown is the accelerated formation of this peptide fragment in hair containing low levels of copper absorbed from hair during washing with tap water containing copper ions. Transmission electron microscopy (TEM) X-ray energy dispersive spectroscopy (XEDS) studies indicate copper is located in the sulphur-poor endo-cuticle region, a region where the S100A3 protein is concentrated. A mechanism for formation of this peptide fragment is proposed in addition to the possible role of lipids in UV oxidation. A shampoo and conditioner containing chelants (EDDS in shampoo and histidine in conditioner) is shown to reduce copper uptake from tap water and reduce protein loss and formation of S100A3 protein fragment. In addition, the long-term consequences of UV oxidation and additional damage induced by copper are illustrated in a fourmonth wear study where hair was treated with a consumer relevant protocol of hair colouring treatments, UV exposure and regular shampoo and conditioning. CONCLUSIONS: The role of copper in accelerating UV damage to hair has been demonstrated as well as the ability of chelants such as EDDS and histidine in shampoo and conditioner products to reduce this damage.
    Creator/Author:
    McComb, D. W.; Marsh, J. M.; Sun, Y.; Chaudhary, T.; Flagler, M. J.; Coderch, L.; Mamak, M.; Greis, Kenneth D.; Rubio, L.; Williams, R. E. A., and Davis, M. G.
    Submitter:
    Kenneth Greis
    Date Uploaded:
    03/03/2017
    Date Modified:
    04/07/2017
    Date Created:
    2015-04
    License:
    All rights reserved

  • Air Monitoring for East Liverpool East Elementary School - CARES Data Set 4

    Type:
    Dataset
    Description/Abstract:
    This data set and accompanying files represents air monitoring data collected by the Environmental Protection Agency from 2009-08-12 to 2012-01-28 at the East Liverpool East Elementary School, in East Liverpool, Ohio (40.635093 , -80.545558). The variables of interest were the amount of manganese and lead in the air measured as PM10 particle size. The visualizations were created from monthly averages for the concentration of airborne manganese. The data was collected using the TO-15 collection systems for air monitoring device. (reference -  https://www3.epa.gov/air/sat/pdfs/VocTechdocwithappendix1209.pdf) The files included are: The raw data - EastLiverpoolEastElementarySchool_Raw.csv . Aggregated monthly averages of the raw data - EastLiverpoolEastElementarySchool_Processed.csv. How the raw data are processed into monthly averages - Marietta_EastLiverpoolEastElementarySchool_WorkingFile.xlsx. How the video is generated- EastLiverpoolEastElementarySchool.ppt. Video - EastLiverpoolEastElementarySchool- generated from EastLiverpoolEastElementarySchool.ppt.
    Creator/Author:
    Yao, Zhiyuan; Hilbert, Tim, and Haynes, Erin
    Submitter:
    Amy Koshoffer
    Date Uploaded:
    10/27/2018
    Date Modified:
    07/11/2019
    Date Created:
    2009-08-12 to 2012-01-28
    License:
    CC0 1.0 Universal

  • Air Monitoring for East Liverpool Water Treatment Plant - CARES Data Set 5

    Type:
    Dataset
    Description/Abstract:
    This data set and accompanying files represents air monitoring data collected by the Environmental Protection Agency from 2009-08-12 to 2012-01-28 at the East Liverpool Water Treatment Plant, in East Liverpool, Ohio (40.639501 , -80.523561). The variables of interest were the amount of manganese and lead in the air measured as PM10 particle size. The visualizations were created from monthly averages for the concentration of airborne manganese The data was collected using the TO-15 collection systems for air monitoring device. (reference -  https://www3.epa.gov/air/sat/pdfs/VocTechdocwithappendix1209.pdf) The files included are: The raw data - EastLiverpool_WaterTreatmentPlant_Raw.csv . Aggregated monthly averages of the raw data - EastLiverpool_WaterTreatmentPlant_Processed.csv. How the raw data are processed into monthly averages - Marietta_EastLiverpool_WaterTreatmentPlant_WorkingFile.xlsx. How the video is generated- EastLiverpool_WaterTreatmentPlant.ppt. Video - EastLiverpool_WaterTreatmentPlant- generated from EastLiverpool_WaterTreatmentPlant.ppt.
    Creator/Author:
    Yao, Zhiyuan; Hilbert, Tim, and Haynes, Erin
    Submitter:
    Amy Koshoffer
    Date Uploaded:
    10/27/2018
    Date Modified:
    07/11/2019
    Date Created:
    2009-01 to 2018-04
    License:
    CC0 1.0 Universal

  • Air Monitoring for Marietta Ohio Valley Educational Service Center - CARES Data Set 3

    Type:
    Dataset
    Description/Abstract:
    This data set and accompanying files represents air monitoring data collected by the Environmental Protection Agency from 2009-08-17 to 2012-02-25 at the Ohio Valley Educational Service Center in Marietta, Ohio (39.443477 , -81.452199). The variables of interest were the amount of manganese and lead in the air measured as PM10 particle size. The visualizations were created from monthly averages for the concentration of airborne manganese The data was collected using the TO-15 collection systems for air monitoring device. (reference -  https://www3.epa.gov/air/sat/pdfs/VocTechdocwithappendix1209.pdf) The files included are: The raw data - Marietta_OhioValleyEducationalServiceCenter_Raw.csv . Aggregated monthly averages of the raw data - Marietta_OhioValleyEducationalServiceCenter_Processed.csv. How the raw data are processed into monthly averages - Marietta_OhioValleyEducationalServiceCenter_WorkingFile.xlsx. How the video is generated- Marietta_OhioValleyEducationalServiceCenter.ppt. Video - Marietta_OhioValleyEducationalServiceCenter - generated from Marietta_OhioValleyEducationalServiceCenter.ppt.
    Creator/Author:
    Yao, Zhiyuan; Hilbert, Tim, and Haynes, Erin
    Submitter:
    Amy Koshoffer
    Date Uploaded:
    10/27/2018
    Date Modified:
    07/11/2019
    Date Created:
    2009-08-17 to 2012-02-25
    License:
    CC0 1.0 Universal

  • Air Monitoring for Marietta Warren Elementary School - CARES Data Set 2

    Type:
    Dataset
    Description/Abstract:
    This data set and accompanying files represents air monitoring data collected by the Environmental Protection Agency from 2009-08-17 to 2012-02-25 at the Warren Elementary School in Marietta, Ohio (39.393536, -81.554015). The variables of interest were the amount of manganese and lead in the air measured as PM10 particle size. The visualizations were created from monthly averages for the concentration of airborne manganese. The data was collected using the TO-15 collection systems for air monitoring device. (reference -  https://www3.epa.gov/air/sat/pdfs/VocTechdocwithappendix1209.pdf) The files included are: The raw data - Marietta_WarrenElementraySchool_Raw.csv . Aggregated monthly averages of the raw data - Marietta_WarrenElementraySchool_Processed.csv. How the raw data are processed into monthly averages - Marietta_WarrenElementraySchool_WorkingFile.xlsx. How the video is generated- Marietta_WarrenElementraySchool.ppt. Video - Marietta_WarrenElementraySchool.mp4 - generated from Marietta_WarrenElementraySchool.ppt.
    Creator/Author:
    Erin Hayes; Zhiyuan Yao, and Tim Hilbert
    Submitter:
    Amy Koshoffer
    Date Uploaded:
    10/26/2018
    Date Modified:
    07/11/2019
    Date Created:
    2009-08-17 to 2012-02-25
    License:
    CC0 1.0 Universal

  • Apolipoprotein A-II-mediated Conformational Changes of Apolipoprotein A-I in Discoidal High Density Lipoproteins

    Type:
    Article
    Description/Abstract:
    Background: Role of apolipoprotein (apo) A-II on metabolism of high density lipoproteins (HDLs) is unknown. Results: Conformational changes of apoA-I, the major apolipoprotein of HDL, caused by apoA-II in discoidal HDL are confined to two regions of apoA-I. Conclusion: Interactions between the two major apolipoproteins in discoidal HDL are site specific. Significance: Functional implications of HDL complexes will significantly benefit from such structural information.
    Creator/Author:
    Gauthamadasa, Kekulawalage; Vaitinadin, Nataraja Sarma; Homan, Reyn; Macha, Stephen; Dresman, James L.; D. Silva, R. A. Gangani, and Greis, Kenneth D.
    Submitter:
    Kenneth Greis
    Date Uploaded:
    03/01/2017
    Date Modified:
    04/07/2017
    Date Created:
    2012-03
    License:
    All rights reserved

  • Cardiac Metabolic Pathways Affected in the Mouse Model of Barth Syndrome

    Type:
    Article
    Description/Abstract:
    Cardiolipin (CL) is a mitochondrial phospholipid essential for electron transport chain (ETC) integrity. CL-deficiency in humans is caused by mutations in the tafazzin (Taz) gene and results in a multisystem pediatric disorder, Barth syndrome (BTHS). It has been reported that tafazzin deficiency destabilizes mitochondrial respiratory chain complexes and affects supercomplex assembly. The aim of this study was to investigate the impact of Taz-knockdown on the mitochondrial proteomic landscape and metabolic processes, such as stability of respiratory chain supercomplexes and their interactions with fatty acid oxidation enzymes in cardiac muscle. Proteomic analysis demonstrated reduction of several polypeptides of the mitochondrial respiratory chain, including Rieske and cytochrome c1 subunits of complex III, NADH dehydrogenase alpha subunit 5 of complex I and the catalytic core-forming subunit of F0F1-ATP synthase. Taz gene knockdown resulted in upregulation of enzymes of folate and amino acid metabolic pathways in heart mitochondria, demonstrating that Tazdeficiency causes substantive metabolic remodeling in cardiac muscle. Mitochondrial respiratory chain supercomplexes are destabilized in CL-depleted mitochondria from Taz knockdown hearts resulting in disruption of the interactions between ETC and the fatty acid oxidation enzymes, very long-chain acyl-CoA dehydrogenase and long-chain 3-hydroxyacylCoA dehydrogenase, potentially affecting the metabolic channeling of reducing equivalents between these two metabolic pathways. Mitochondria-bound myoglobin was significantly reduced in Taz-knockdown hearts, potentially disrupting intracellular oxygen delivery to the oxidative phosphorylation system. Our results identify the critical pathways affected by the Taz-deficiency in mitochondria and establish a future framework for development of therapeutic options for BTHS.
    Creator/Author:
    Haffey, Wendy D.; Mandala, Satish K.; Purevjav, Enkhsalkhan; Huang, Yan; Javadov, Sabzali; Greis, Kenneth D.; Powers, Corey; Strauss, Arnold W.; Towbin, Jeffrey A., and Khuchua, Zaza
    Submitter:
    Kenneth Greis
    Date Uploaded:
    03/03/2017
    Date Modified:
    04/07/2017
    Date Created:
    2015-06
    License:
    All rights reserved

  • Cardiac myosin binding protein-C is a potential diagnostic biomarker for myocardial infarction

    Type:
    Article
    Description/Abstract:
    Cardiac myosin binding protein-C (cMyBP-C) is a thick filament assembly protein that stabilizes sarcomeric structure and regulates cardiac function; however, the profile of cMyBP-C degradation after myocardial infarction (MI) is unknown. We hypothesized that cMyBP-C is sensitive to proteolysis and is specifically increased in the bloodstream post-MI in rats and humans. Under these circumstances, elevated levels of degraded cMyBP-C could be used as a diagnostic tool to confirm MI. To test this hypothesis, we first established that cMyBP-C dephosphorylation is directly associated with increased degradation of this myofilament protein, leading to its release in vitro. Using neonatal rat ventricular cardiomyocytes in vitro, we were able to correlate the induction of hypoxic stress with increased cMyBP-C dephosphorylation, degradation, and the specific release of N′-fragments. Next, to define the proteolytic pattern of cMyBP-C post-MI, the left anterior descending coronary artery was ligated in adult male rats. Degradation of cMyBP-C was confirmed by a reduction in total cMyBP-C and the presence of degradation products in the infarct tissue. Phosphorylation levels of cMyBP-C were greatly reduced in ischemic areas of the MI heart compared to non-ischemic regions and sham control hearts. Post-MI plasma samples from these rats, as well as humans, were assayed for cMyBP-C and its fragments by sandwich ELISA and immunoprecipitation analyses. Results showed significantly elevated levels of cMyBP-C in the plasma of all post-MI samples. Overall, this study suggests that cMyBP-C is an easily releasable myofilament protein that is dephosphorylated, degraded and released into the circulation post-MI. The presence of elevated levels of cMyBP-C in the blood provides a promising novel biomarker able to accurately rule in MI, thus aiding in the further assessment of ischemic heart disease.
    Creator/Author:
    Muthusamy, Saminathan; Nair, Nandini; McElligott, Andrew; Martin, Jody L.; Govindan, Suresh; Gongora, Enrique; henderson, Kyle K.; Greis, Kenneth D.; Barefield, David; Luther, Pradeep K.; Sadayappan, Sakthivel, and Winegrad, Saul
    Submitter:
    Kenneth Greis
    Date Uploaded:
    03/03/2017
    Date Modified:
    04/07/2017
    Date Created:
    2012-01
    License:
    All rights reserved

  • Clot On a Suture Dataset: Human clots in porcine plasma, Plasma Only

    Type:
    Dataset
    Description/Abstract:
    A raw dataset produced using the clot on a suture experimental set up in the Holland lab (previously published in Bader 2015 & other articles). Data gathered and further analyzed using MATLAB 2012b.
    Creator/Author:
    Huang, Shenwen; Holland, Christy, and Shekhar, Himanshu
    Submitter:
    Shenwen Huang
    Date Uploaded:
    01/05/2017
    Date Modified:
    05/08/2017
    License:
    Public Domain Mark 1.0

  • Clot On a Suture Dataset: Human clots in porcine plasma, rt-PA (3.15 ug/ml)

    Type:
    Dataset
    Description/Abstract:
    A raw dataset produced using the clot on a suture experimental set up in the Holland lab (previously published in Bader 2015 & other articles). Data gathered and further analyzed using MATLAB 2012b.
    Creator/Author:
    Huang, Shenwen; Holland, Christy, and Shekhar, Himanshu
    Submitter:
    Shenwen Huang
    Date Uploaded:
    01/05/2017
    Date Modified:
    05/08/2017
    License:
    Public Domain Mark 1.0

  • Clot On a Suture Dataset: Human clots in porcine plasma, rt-PA (3.15 ug/ml), Definity, & US

    Type:
    Dataset
    Description/Abstract:
    A raw dataset produced using the clot on a suture experimental set up in the Holland lab (previously published in Bader 2015 & other articles). Data gathered and further analyzed using MATLAB 2012b.
    Creator/Author:
    Huang, Shenwen; Holland, Christy, and Shekhar, Himanshu
    Submitter:
    Shenwen Huang
    Date Uploaded:
    12/22/2016
    Date Modified:
    05/08/2017
    License:
    Public Domain Mark 1.0

  • Clot On a Suture Dataset: Porcine clots in human plasma, Plasma Only

    Type:
    Dataset
    Description/Abstract:
    A raw dataset produced using the clot on a suture experimental set up in the Holland lab (previously published in Bader 2015 & other articles). Data gathered and further analyzed using MATLAB 2012b.
    Creator/Author:
    Huang, Shenwen; Holland, Christy, and Shekhar, Himanshu
    Submitter:
    Shenwen Huang
    Date Uploaded:
    01/05/2017
    Date Modified:
    05/08/2017
    License:
    Public Domain Mark 1.0

  • Clot On a Suture Dataset: Porcine clots in human plasma, rt-PA (3.15 ug/ml)

    Type:
    Dataset
    Description/Abstract:
    A raw dataset produced using the clot on a suture experimental set up in the Holland lab (previously published in Bader 2015 & other articles). Data gathered and further analyzed using MATLAB 2012b.
    Creator/Author:
    Huang, Shenwen; Holland, Christy, and Shekhar, Himanshu
    Submitter:
    Shenwen Huang
    Date Uploaded:
    01/05/2017
    Date Modified:
    05/08/2017
    License:
    Public Domain Mark 1.0

  • Clot On a Suture Dataset: Porcine clots in human plasma, rt-PA (3.15 ug/ml), Definity, & US

    Type:
    Dataset
    Description/Abstract:
    A raw dataset produced using the clot on a suture experimental set up in the Holland lab (previously published in Bader 2015 & other articles). Data gathered and further analyzed using MATLAB 2012b.
    Creator/Author:
    Huang, Shenwen; Holland, Christy, and Shekhar, Himanshu
    Submitter:
    Shenwen Huang
    Date Uploaded:
    05/05/2017
    Date Modified:
    05/08/2017
    License:
    Public Domain Mark 1.0

  • Clot On a Suture Dataset: Porcine, Plasma Only

    Type:
    Dataset
    Description/Abstract:
    A raw dataset produced using the clot on a suture experimental set up in the Holland lab (previously published in Bader 2015 & other articles). Data gathered and further analyzed using MATLAB 2012b.
    Creator/Author:
    Huang, Shenwen; Holland, Christy, and Shekhar, Himanshu
    Submitter:
    Shenwen Huang
    Date Uploaded:
    12/22/2016
    Date Modified:
    05/08/2017
    License:
    Public Domain Mark 1.0

  • Clot On a Suture Dataset: Porcine, rt-PA (3.15 ug/ml)

    Type:
    Dataset
    Description/Abstract:
    A raw dataset produced using the clot on a suture experimental set up in the Holland lab (previously published in Bader 2015 & other articles). Data gathered and further analyzed using MATLAB 2012b.
    Creator/Author:
    Huang, Shenwen; Holland, Christy, and Shekhar, Himanshu
    Submitter:
    Shenwen Huang
    Date Uploaded:
    12/22/2016
    Date Modified:
    05/08/2017
    License:
    Public Domain Mark 1.0

  • Clot On a Suture Dataset: Porcine, rt-PA (3.15 ug/ml), Definity, & US

    Type:
    Dataset
    Description/Abstract:
    A raw dataset produced using the clot on a suture experimental set up in the Holland lab (previously published in Bader 2015 & other articles). Data gathered and further analyzed using MATLAB 2012b.
    Creator/Author:
    Huang, Shenwen; Holland, Christy, and Shekhar, Himanshu
    Submitter:
    Shenwen Huang
    Date Uploaded:
    12/22/2016
    Date Modified:
    05/08/2017
    License:
    Public Domain Mark 1.0

  • Developing Best Practices to Address LGBTQ+ Health Disparities - Panelists 3 & 4 - UC Data Day

    Type:
    Document
    Description/Abstract:
    This presentation represents Panelists 3 and 4 as a joint presentation and This talk was the third panelist in the Health Equities and Disparities Session for the 4th Annual UC Data Day Conference hosted by UC Libraries. Joint Talk with Dr. Pickle and Stef Murwsky – Title: Developing Best Practices to Address LGBTQ and Health Disparities Sarah Pickle, MD (she/her/hers), Associate Professor, University of Cincinnati Department of Family and Community Medicine. Dr. Pickle and her colleagues are studying best practices for training future generations of health care professionals in transgender medicine. University of Cincinnati College of Medicine is one of the only US Medical Schools to have a nationally published, dedicated transgender medicine curriculum. Stef Murawsky, MA, WGSS, Ph.D. Candidate, Sociology Pronouns: they/them/theirs University of Cincinnati Department of Sociology I am currently completing a qualitative dissertation that explores transgender patient experiences of navigating and managing a stigmatized gender identity in biomedical contexts. I plan to generate a critical analysis of stigma in healthcare that demonstrates how structural, interpersonal and individual level transgender healthcare experiences are gendered and racialized.
    Creator/Author:
    Murawsky, Stef and Pickel, Sara
    Submitter:
    Amy Koshoffer
    Date Uploaded:
    04/12/2019
    Date Modified:
    04/12/2019
    Date Created:
    2019-04-01
    License:
    Attribution-NonCommercial 4.0 International

  • Development and Validation of a Whole-Cell Inhibition Assay for Bacterial Methionine Aminopeptidase by Surface-Enhanced Laser Desorption Ionization–Time of Flight Mass Spectrometry

    Type:
    Article
    Description/Abstract:
    Bacterial methionine aminopeptidase (MAP) is a protease that removes methionine from the N termini of newly synthesized bacterial proteins after the peptide deformylase enzyme cleaves the formyl group from the initiator formylmethionine. MAP is an essential bacterial gene product and thus represents a potential target for therapeutic intervention. A fundamental challenge in the antibacterial drug discovery field is demonstrating conclusively that compounds with in vitro enzyme inhibition activity produce the desired antibacterial effect by interfering with the same target in whole bacterial cells. One way to address the activity of inhibitor compounds is by profiling cellular biomarkers in whole bacterial cells using compounds that are known inhibitors of a particular target. However, in the case of MAP, no specific inhibitors were available for such studies. Instead, a genetically attenuated MAP strain was generated in which MAP expression was placed under the control of an inducible arabinose promoter. Thus, MAP inhibition in whole cells could be mimicked by growth in the absence of arabinose. This genetically attenuated strain was used as a benchmark for MAP inhibition by profiling whole-cell lysates for unprocessed proteins using surface-enhanced laser desorption ionization–time of flight mass spectrometry (MS). Eight proteins between 4 and 14 kDa were confirmed as being unprocessed and containing the initiator methionine by adding back purified MAP to the preparations prior to MS analysis. Upon establishing these unprocessed proteins as biomarkers for MAP inhibition, the assay was used to screen small-molecule chemical inhibitors of purified MAP for whole-cell activity. Fifteen compound classes yielded three classes of compound with whole-cell activity for further optimization by chemical expansion. This report presents the development, validation, and implementation of a whole-cell inhibition assay for MAP.
    Creator/Author:
    Siehnel, Richard; Howard, Jeremy; Zhou, Songtao; Layh-Schmitt, Gerlinde; Greis, Kenneth D.; Curnow, Alan, and Klanke, Chuck
    Submitter:
    Kenneth Greis
    Date Uploaded:
    03/03/2017
    Date Modified:
    04/07/2017
    Date Created:
    2005-09
    License:
    All rights reserved