Paraprofessional education candidates (associate degree level) and pre-service teachers participated in Visible Thinking (Ritchart, Church & Morrison, 2011) activities during undergraduate coursework to understand, inform, and then reflect on current topics in education while forming professional identities. The Visible Thinking process and reflections will be shared relating to professional development and inquiry.
Robert Ross brings to light ninety-eight foundational texts of Khoesan political thought and highlights the voices of the Khoesan people and their inspiring history of resistance in the face of colonial oppression.
This was the morning keynote for the 4th Annual UC Data Day Conference hosted by UC Libraries.
The keynote presenter was Amanda J. Wilson, Head, National Network Coordinating Office, National Library of Medicine, All of Us Research Program partner
Fungi in the genus Pneumocystis are the cause of a potentially life threatening
pneumonia, Pneumocystis pneumonia (PCP). The understanding of the lifecycle, metabolism, and drug development has been hindered due to a lack of a long term in vitro culture system. Unlike most other fungi, members of the genus Pneumocystis do not appear to synthesize the major fungal sterol, ergosterol. However, genome scans and in vitro assays suggest the presence of functional genes involved in a sterol pathway. One of the goals of this work was to characterize the P. carinii sterol enzyme, lanosterol synthase (Erg7p), an essential enzyme of the sterol pathway. The activity of P. carinii Erg7p was assessed by heterologous expression of P. carinii Erg7p in a Saccharomyces cerevisiae Erg7p null mutant. Growth rates and lanosterol production were similar between S. cerevisiae expressing the P. carinii enzyme and S. cerevisiae expressing its own Erg7p under the same conditions, indicating that not only does P. carinii produce a functional Erg7p, but also that the enzyme functionally complements the S. cerevisiae enzyme. Western blotting and fluorescent localization studies revealed that P. carinii Erg7p localizes to lipid particles in S. cerevisiae as does S. cerevisiae Erg7p. A novel finding of these studies, was that P. carinii contains lipid particles, and that P. carinii Erg7p localizes to lipid particles in P. carinii. These studies indicate that P. carinii Erg7p functions similar to the S. cerevisiae enzyme, and may perform a similar function in P. carinii.
Biochemical analyses of sterols within the membranes of P. carinii have shown that it utilizes cholesterol rather than ergosterol as its bulk sterol. However, P. carinii does not appear to synthesize cholesterol from a de novo pathway, but rather scavenges
exogenous sterols from its mammalian host. S. cerevisiae is induced to undergo sterol
scavenging under anaerobic conditions. Consequently, another goal of this work was to provide information on the effect of O2 on sterol biosynthesis and sterol scavenging by P. carinii. ATP measurements revealed that the viability of P. carinii is severely decreased when maintained under hypoxic conditions, and this decrease correlated with an increase in drug susceptibility. We show that uptake of exogenous cholesterol by P. carinii occurred under normal O2 tensions, indicating that sterol scavenging is not limited to anaerobic conditions. Microarray analysis indicated that hypoxic maintenance of P. carinii resulted in decreased transcription of several genes involved in sterol and lipid biosynthesis suggesting that while hypoxic conditions down-regulated genes involved in sterol biosynthesis, down-regulation of sterol biosynthesis is not a requirement for sterol scavenging in P. carinii. The ability of P. carinii to scavenge exogenous sterols under normal O2 tensions at which the sterol pathway is unaffected provides evidence that sterol scavenging may be the primary means that P. carinii utilizes to obtain its sterols.
Over the past 20 years the internet and technology boom has transformed education teaching approaches and techniques. The introduction of online courses has brought remote communication and collaboration to the center of the discussion. It’s no longer an option for faculty and staff to put their head in the sand and ignore this technological revolution. Even traditional courses and student service offices must engage in communicating and collaborating with students where they are, both in person and online. The presentation will include a walk-through of Professor Theis’ history of integrating cloud-based technology tools in his courses. There have been many ups and downs, successes and failures. He’ll then explain how he’s using one primary application called Slack to engage his students and prepare them for similar tools they’ll be expected to use in the workplace. He’ll discuss a step-by-step process to help higher education professionals determine if, when, and how to introduce or integrate industry-related cloud-based tools into their work with students. We’ll walk through the questions we need to ask when making these decisions. How is my student demographic currently communicating with their faculty and with each other? What are the standard communication and collaboration tools in the industry they may enter in the next few years? Are there processes or tools that I’m currently using that can be easily replaced with a cloud-based technology? Which tools are free for practitioners and students? Finally, he’ll help attendees create a plan and timeline for integrating one or two cloud-based technologies into their work.