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  • Purification and Characterization an Extracellular Phosphoglycan from Leishmania donovani

    Article thumbnail: Purification and Characterization an Extracellular Phosphoglycan from Leishmania donovani
    Type:
    Article
    Description/Abstract:
    An extracellular phosphoglycan (exPG), present in the culturem edium of the promastigote form L oefi shmania donovani, was purified and structurally characterized. The purification scheme included ethanol precipitation of the culture medium, anion exchange chromatography, hydrophobic chromatography on phenyl-Sepharose, and preparative polyacrylamgeild e electrophoresis. Structural analysis by ‘H-’H NMR, methylation linkage analysis, and glycosidase digestion revealed that the exPG consisted of thfoel lowing structure: (CAP)+[P04-6Galp@1-4Manpal]lo-11-POr6GalpB1-4Man. The capw as found to be ones eovf eral small, neutral oligosaccharides, the most abundant of which was the trisaccharide Galp@l-4(Manpal-2)Man. The results indicated structural analogy to the cellular-derived lipophosphoglycan (LPG) from L. donovani. The important exceptions are a lacko f the lipid anchor, the entire phosphosaccharide core, and several of the repeating disaccharide units. Although the function of exPGis presently unknowni,t may play a protective role for the promastigote in the insect vector or during infection of a mammalian host
    Creator/Author:
    Turco, Salvatore J.; Thomas, Jerry R.; Homans, Steven W.; Ferguson, Michael A.; McConville, Malcolm J., and Greis, Kenneth D.
    Submitter:
    Kenneth Greis
    Date Uploaded:
    03/01/2017
    Date Modified:
    04/07/2017
    Date Created:
    1992-04
    License:
    All rights reserved