Data of monarchs subjected to righting response orientation trials under different artificial magnetic fields pre- and post-overwintering cold treatment.
The Auditory Brainstem Response (ABR) to gated tone burst of five frequencies (0.125, 0.5, 4, 8, 10 kHz) as well as broadband click stimuli were recorded in two cheetahs, one male and one female. We report, for the first time, hearing estimates from two cheetahs. Provided are the raw data files in PDF format and RPT format which is the file format used by the Intelligent Hearing System Universal Smart Box for analysis and to mark waves in order to obtain amplitude and latency of waves. Data were obtained for the female cheetah on February 1, 2024 and for the male cheetah on March 26, 2024 from the Cincinnati Zoo and Botanical Gardens.
This research project investigates and improves the processes of native seed collection and native plant cultivation specific to the Ohio region. The overarching goal is advancing urban biodiversity initiatives, focused on flora indigenous to the Cincinnati area. The project delves into current research gaps and challenges associated with native seed collection and cultivation methodologies. Additionally, this research explores the potential ecological benefits and challenges associated with integrating native plant species into urban landscapes. This project researches 13 plant species in this context. The target outcomes of this research include a refined understanding of native plant adaptation to urban settings, improved conservation strategies, and actionable guidelines for urban planners and horticulturalists. This research contributes knowledge to the ongoing efforts of promoting biodiversity within urban landscapes, fostering a more sustainable coexistence between human developments and Ohio's native flora. The final result of this project is a field guide with sections on plant identification, seed harvesting, seed storage, cultivation needs, and common ecoregions for each plant.
Human iPSCs (TkDA cell-line) were differentiated on laminin coated plates into endoderm by treatment of Activin and BMP, then treated with FGF4 and CHIR to further differentiate into posterior foregut. The cells were embedded into Matrigel droplets and cultured in Advanced DMEM. Droplet media was collected for ELISA to measure Albumin concentrations. The droplets were collected for histology and RNA isolation to test for AFP, ALB, and HBG1 genes. These methods resulted in the creation of a novel culture system containing both hepatic and hematopoietic lineage cells to model developing fetal liver.
