SNU-407 colorectal cancer cells were treated with 300nM MRTX1133 for 0, 24, 48, and 72 hours before lysis and loading onto 7.5% SDS-PAGE gels. Gels were transferred to nitrocellulose membranes and cut at the 95kDa and just below the 52 kDa molecular weight (MW) markers. Membranes were then probed for proteins that fell within the MW and evaluated for change in comparison to the 0h control.
LS513 cells were treated with a combination of varying concentrations of MRTX1133 with varying concentrations of either afatinib, sapitinib, or pelitinib for 72 hours. Absorbances were normalized to DMSO control for % viability. The attached files were compiled in data format from n=2 data sets (6 data points total for each combination) and uploaded to SynergyFinder+ with % viability chosen as response.