Matrices of DNA sequences used to generate the phylogeny of Aniba rosiodora and related species (Lauraceae) presented in the manuscript entitled "Chemical and genotypic variations in Aniba species from the Amazonian forest"
The Auditory Brainstem Response (ABR) to gated tone burst of five frequencies (0.125, 0.5, 4, 8, 10 kHz) as well as broadband click stimuli were recorded in two cheetahs, one male and one female. We report, for the first time, hearing estimates from two cheetahs. Provided are the raw data files in PDF format and RPT format which is the file format used by the Intelligent Hearing System Universal Smart Box for analysis and to mark waves in order to obtain amplitude and latency of waves. Data were obtained for the female cheetah on February 1, 2024 and for the male cheetah on March 26, 2024 from the Cincinnati Zoo and Botanical Gardens.
Raw data for: Siers, S.R., Mungaray, J.-C., Kastner, M. & Jayne, B.C. (2025) Hard to swallow: scaling relationships between the size of avian prey and the overall size and maximal gape of brown treesnakes. Ecology and Evolution (in revision). (BCJ corresponding author)
Proteomic analysis of biological samples in disease models or therapeutic intervention studies requires the ability to detect and identify biologically relevant proteins present in relatively low concentrations. The detection and analysis of these low-level proteins is hindered by the presence of a few proteins that are expressed in relatively high concentrations. In the case of muscle tissue, highly abundant structural proteins, such as actin, myosin, and tropomyosin, compromise the detection and analysis of more biologically relevant proteins. We have developed a practical protocol which exploits high-pH extraction to reduce or remove abundant structural proteins from skeletal muscle crude membrane preparations in a manner suitable for two dimensional gel electrophoresis. An initial whole-cell muscle lysate is generated by homogenization of powdered tissue in Tris-base. This lysate is subsequently partitioned into a supernatant and pellet containing the majority of structural proteins. Treatment of the pellet with high-pH conditions effectively releases structural proteins from membrane compartments which are then removed through ultracentrifugation. Mass spectrometric identification shows that the majority of protein spots reduced or removed by high-pH treatment were contractile proteins or contractile-related proteins. Removal of these proteins enabled successful detection and identification of minor proteins. Structural protein removal also results in significant improvement of gel quality and the ability to load higher amounts of total protein for the detection of lower abundant protein classes.
Greater Caribbean manatee (Trichechus manatus manatus) pool recordings from the Caribbean Manatee Conservation Center in Bayamon, Puerto Rico including location and depth of recordings as well as recordings from the natural protected habitat of Laguna del Condado, San Juan, Puerto Rico which includes the GPS coordinates in the recording title. All recordings obtained using omnidirectional hydrophones with H1 recorder (Model SQ26-H1; Cetacean Research Technology, 2007) and are in .wav format.
Greater Caribbean manatee (Trichechus manatus manatus) pool recordings from the Caribbean Manatee Conservation Center in Bayamon, Puerto Rico including location and depth of recordings as well as recordings from the natural protected habitat of Laguna del Condado, San Juan, Puerto Rico which includes the GPS coordinates in the recording title. All recordings obtained using omnidirectional hydrophones with H1 recorder (Model SQ26-H1; Cetacean Research Technology, 2007) and are in .wav format.
Greater Caribbean manatee (Trichechus manatus manatus) pool recordings from the Caribbean Manatee Conservation Center in Bayamon, Puerto Rico including location and depth of recordings as well as recordings from the natural protected habitat of Laguna del Condado, San Juan, Puerto Rico which includes the GPS coordinates in the recording title. All recordings obtained using omnidirectional hydrophones with H1 recorder (Model SQ26-H1; Cetacean Research Technology, 2007) and are in .wav format.
Greater Caribbean manatee (Trichechus manatus manatus) pool recordings from the Caribbean Manatee Conservation Center in Bayamon, Puerto Rico including location and depth of recordings as well as recordings from the natural protected habitat of Laguna del Condado, San Juan, Puerto Rico which includes the GPS coordinates in the recording title. All recordings obtained using omnidirectional hydrophones with H1 recorder (Model SQ26-H1; Cetacean Research Technology, 2007) and are in .wav format.
Human iPSCs (TkDA cell-line) were differentiated on laminin coated plates into endoderm by treatment of Activin and BMP, then treated with FGF4 and CHIR to further differentiate into posterior foregut. The cells were embedded into Matrigel droplets and cultured in Advanced DMEM. Droplet media was collected for ELISA to measure Albumin concentrations. The droplets were collected for histology and RNA isolation to test for AFP, ALB, and HBG1 genes. These methods resulted in the creation of a novel culture system containing both hepatic and hematopoietic lineage cells to model developing fetal liver.
This research shows the environmental benefits of implementing floating garden designs in ponds and lakes as floating treatment wetlands (FTWs). Research conducted by Virginia Tech, Rutgers, Floating Island International, and the International Institute for Sustainable Development indicates successful remediation and removal of nutrient runoff from agricultural production, wastewater treatment, and human and animal waste. Plants with different rooting depths and surface areas can phytoaccumulate phosphorus and nitrogen compounds. Like wetlands, FTWs create an environment for microbial life around the rhizosphere of plants and the island substrate, generating colonies of bacteria called biofilm that digest nutrients from water or sediment. Natural wetland habitats, one of humanity's most efficient biofilters, have been destroyed around the United States to make room for farmland and cities. This research has been aggregated to fuel an FTW design on a small farm in Waynesville, Ohio, and other lakes and ponds for people that this research may inspire.
Organovanadium compounds have been shown to be insulin sensitizers in vitro and in vivo. One potential biochemical mechanism for insulin sensitization by these compounds is that they inhibit protein tyrosine phosphatases (PTPs) that negatively regulate insulin receptor activation and signaling. In this study, bismaltolato oxovanadium (BMOV), a potent insulin sensitizer, was shown to be a reversible, competitive phosphatase inhibitor that inhibited phosphatase activity in cultured cells and enhanced insulin receptor activation in vivo. NMR and X-ray crystallographic studies of the interaction of BMOV with two different phosphatases, HCPTPA (human low molecular weight cytoplasmic protein tyrosine phosphatase) and PTP1B (protein tyrosine phosphatase 1B), demonstrated uncomplexed vanadium (VO ) in the active site. Taken together, these findings support phosphatase inhibition as a mechanism for insulin sensitization by BMOV 4 and other organovanadium compounds and strongly suggest that uncomplexed vanadium is the active component of these compounds.
Data of monarchs subjected to righting response orientation trials under different artificial magnetic fields pre- and post-overwintering cold treatment.
This research project investigates and improves the processes of native seed collection and native plant cultivation specific to the Ohio region. The overarching goal is advancing urban biodiversity initiatives, focused on flora indigenous to the Cincinnati area. The project delves into current research gaps and challenges associated with native seed collection and cultivation methodologies. Additionally, this research explores the potential ecological benefits and challenges associated with integrating native plant species into urban landscapes. This project researches 13 plant species in this context. The target outcomes of this research include a refined understanding of native plant adaptation to urban settings, improved conservation strategies, and actionable guidelines for urban planners and horticulturalists. This research contributes knowledge to the ongoing efforts of promoting biodiversity within urban landscapes, fostering a more sustainable coexistence between human developments and Ohio's native flora. The final result of this project is a field guide with sections on plant identification, seed harvesting, seed storage, cultivation needs, and common ecoregions for each plant.
An extracellular phosphoglycan (exPG), present in the culturem edium of the promastigote form L oefi shmania donovani, was purified and structurally characterized. The purification scheme included ethanol precipitation of the culture medium, anion exchange chromatography, hydrophobic chromatography on phenyl-Sepharose, and preparative polyacrylamgeild e electrophoresis. Structural analysis by ‘H-’H NMR, methylation linkage analysis, and glycosidase digestion revealed that the exPG consisted of thfoel lowing structure: (CAP)+[P04-6Galp@1-4Manpal]lo-11-POr6GalpB1-4Man. The capw as found to be ones eovf eral small, neutral oligosaccharides, the most abundant of which was the trisaccharide Galp@l-4(Manpal-2)Man. The results indicated structural analogy to the cellular-derived lipophosphoglycan (LPG) from L. donovani. The important exceptions are a lacko f the lipid anchor, the entire phosphosaccharide core, and several of the repeating disaccharide units. Although the function of exPGis presently unknowni,t may play a protective role for the promastigote in the insect vector or during infection of a mammalian host
The primary structure of the major surface glycoconjugate of Leishmania donovani parasites, a lipophosphoglycan, has been further characterized. The repeating PO4-6Galp beta 1-4Man disaccharide units, which are a salient feature of the molecule, are shown to terminate with one of several neutral structures, the most abundant of which is the branched trisaccharide Galp beta 1-4(Manp alpha 1-2)Man. The phosphosaccharide core of lipophosphoglycan, which links the disaccharide repeats to a lipid anchor, contains 2 phosphate residues. One of the core phosphates has previously been localized on O-6 of the galactosyl residue distal to the lipid anchor; the second phosphate is now shown to be on O-6 of the mannosyl residue distal to the anchor and to bear an alpha-linked glucopyranosyl residue. Also, the anomeric configuration of the unusual 3-substituted Galf residue in the phosphosaccharide core is established as beta. The complete structure of the core is thus PO4-6Galp alpha 1-6Galp alpha 1-3Galf beta 1-3[Glcp alpha 1-PO4-6]Manp alpha 1-3Manp alpha 1-4GlcN alpha 1-. This further clarification of the structure of lipophosphoglycan may prove beneficial in determining the structure-function relationships of this highly unusual glycoconjugate.
