A presentation and paper delivered at Open Repositories 2012, the 7th International Conference on Open Repositories, held in Edinburgh, Scotland, July 9-13, 2012.
Three part work created for my exhibition at Shirley Jones Gallery in Yellow Springs, Ohio. Features dance treatments from Experimental Television Center, as well as footage from my backyard on Riddle Rd in Cincinnati. The piece was projected onto the store front windows of the gallery.
A video series that walks the viewer through the process of conducting legal research using paper resources.
Video 1 -- Legal Research: Intro
Video 2 -- Legal Research: Define Problem
Video 3 -- Legal Research: Find a Starting Point
Video 4 -- Legal Research: Read Starting Point
Video 5 -- Legal Research: Additional Material
Video 6 -- Legal Research: Validation
Video 7 -- Legal Research: Summary
Organisms in the genus Pneumocystis are ubiquitous, opportunistic pathogenic fungi capable of causing a
lethal pneumonia in immunocompromised mammalian hosts. Pneumocystis spp. are unique members of the
fungal kingdom due to the absence of ergosterol in their cellular membranes. Although these organisms were
thought to obtain cholesterol by scavenging, transcriptional analyses indicate that Pneumocystis carinii encodes
gene homologs involved in sterol biosynthesis. To better understand the sterol pathway in these uncultivable
fungi, yeast deletion strains were used to interrogate the function and localization of P. carinii lanosterol synthase (ERG7). The expression of PcErg7p in an ERG7-null mutant of the yeast Saccharomyces cerevisiae did not alter its growth rate and produced a functional lanosterol synthase, as evidenced by the presence of
lanosterol detected by gas chromatographic analysis in levels comparable to that produced by the yeast enzyme. Western blotting and fluorescence microscopy revealed that, like the S. cerevisiae Erg7p, the PcErg7p localized to lipid particles in yeast. Using fluorescence microscopy, we show for the first time the presence of apparent lipid particles in P. carinii and the localization of PcErg7p to lipid particles in P. carinii. The detection of lipid particles in P. carinii and their association with PcErg7p therein provide strong evidence that the enzyme serves a similar function in P. carinii. Moreover, the yeast heterologous system should be a useful tool for further analysis of the P. carinii sterol pathway.