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- Type:
- Student Work
- Description/Abstract:
- Submitted to the Faculty of the Information Engineering Technology Program in partial fulfillment of the Requirements for the Degree of Bachelor of Science in Information Engineering Technology
- Creator/Author:
- Moore, Nathan
- Submitter:
- CECH Library Service
- Date Uploaded:
- 03/09/2017
- Date Modified:
- 03/09/2017
- Date Created:
- 2001-05-31
- License:
- Attribution-NonCommercial-NoDerivs 4.0 International
-
- Type:
- Student Work
- Description/Abstract:
- Submitted to the Faculty of the Information Engineering Technology Program in Partial Fulfillment of the Requirements for the Degree of Bachelor of Science in Information Engineering Technology
- Creator/Author:
- Brown, Robert P. III
- Submitter:
- CECH Library Service
- Date Uploaded:
- 03/09/2017
- Date Modified:
- 03/09/2017
- Date Created:
- 2001-03-15
- License:
- Attribution-NonCommercial-NoDerivs 4.0 International
-
- Type:
- Student Work
- Description/Abstract:
- Submitted to the Faculty of the Information Engineering Technology Program in Partial Fulfillment of the Requirements for the Degree of Bachelor of Science in Information Engineering Technology
- Creator/Author:
- Bowman, Ralph Stephen
- Submitter:
- CECH Library Service
- Date Uploaded:
- 03/09/2017
- Date Modified:
- 03/09/2017
- Date Created:
- 2001-05-29
- License:
- Attribution-NonCommercial-NoDerivs 4.0 International
-
- Type:
- Student Work
- Description/Abstract:
- Submitted to The faculty of the Information Engineering Technology Program In Partial Fulfillment of the Requirements for The Degree of Bachelor of Science In Information Engineering Technology
- Creator/Author:
- Black, Jeffery R
- Submitter:
- CECH Library Service
- Date Uploaded:
- 03/09/2017
- Date Modified:
- 03/09/2017
- Date Created:
- 2001-03-07
- License:
- Attribution-NonCommercial-NoDerivs 4.0 International
-
- Type:
- Student Work
- Description/Abstract:
- Submitted to the Faculty of the Information Engineering Technology Program in Partial Fulfillment of the Requirements for the Degree of Bachelor of Science in Information Engineering Technology
- Creator/Author:
- Bartels, Joseph and Troxell, Doug
- Submitter:
- CECH Library Service
- Date Uploaded:
- 03/09/2017
- Date Modified:
- 03/09/2017
- Date Created:
- 2001-03-06
- License:
- Attribution-NonCommercial-NoDerivs 4.0 International
-
- Type:
- Article
- Description/Abstract:
- Background: Acid -glucosidase is trafficked to the lysosome by LIMP-2. Results: A unique 11-amino acid sequence on acid -glucosidase was critical for its LIMP-2-dependent targeting to the lysosome. Conclusion: This sequence is essential for oligosaccharide-independent targeting of synthesized acid -glucosidase to the lysosome. Significance: Modification of this sequence has basic/therapeutic implications for Gaucher disease and its comorbidities (e.g. Parkinson disease).
- Creator/Author:
- Grabowski, Gregory A.; Haffey, Wendy D.; Greis, Kenneth D., and Llou, Benjamin
- Submitter:
- Kenneth Greis
- Date Uploaded:
- 03/03/2017
- Date Modified:
- 04/07/2017
- Date Created:
- 2014-09
- License:
- All rights reserved
-
- Type:
- Article
- Description/Abstract:
- Lupus nephritis (LN) is among the main determinants of poor prognosis in systemic lupus erythematosus (SLE). The objective of this study was to 1) isolate and identify proteins contained in the LN urinary protein signature (PS) of children with SLE; 2) assess the usefulness of the PS-proteins for detecting activity of LN over time. Using surface-enhanced or matrix assisted laser desorption/ ionization time of flight mass spectrometry, the proteins contained in the LN urinary PS were identified. They were transferrin (Tf), ceruloplasmin (Cp), α1-acid-glycoprotein (AGP), lipocalintype prostaglandin-D synthetase (L-PGDS), albumin and albumin-related fragments. Serial plasma and urine samples were analyzed using immunonephelometry or ELISA in 98 children with SLE (78% African-American) and 30 controls with juvenile idiopathic arthritis. All urinary PS-proteins were significantly higher with active versus inactive LN or in patients without LN (all p<0.005), and their combined area under the receiver operating characteristic curve was 0.85. As early as 3 months before a clinical diagnosis of worsening LN, significant increases of urinary Tf, AGP (both p < 0.0001) and L-PGDS (p < 0.01) occurred, indicating that these PS-proteins are biomarkers of LN activity and may help anticipate the future course of LN. Systemic Lupus Erythematosus (SLE) is an inflammatory autoimmune disease and lupus nephritis (LN) is one of the main determinants of poor prognosis (1). Currently, LN is gauged by measuring circulating and excreted indicators of renal dysfunction, with supporting information from kidney biopsies. The latter constitute the current standard for diagnosing LN, providing a direct assessment of the presence, severity and activity of LN, and the degree of renal damage (2). Due to the invasive nature of kidney biopsies, clinicians base LN activity and its therapy on the results of urinary protein excretion, urinary sediment, creatinine clearance and serum albumin. These traditional markers are not accurate in assessing whether active LN is present or not, and none of them is predictive, i.e. can anticipate the course of LN. Using Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF MS) technology, we previously identified a LN urinary protein signature (PS), consisting of eight candidate biomarkers at the mass-to-charge ratios (m/z) of 2.763, 22, 23, 44, 56, 79, 100, and 133 kDa (3). In this study, we present the identification of the specific proteins contained in this PS of children with LN. We further assayed plasma and urine samples of SLE patients and controls with juvenile idiopathic arthritis (JIA) to investigate the concurrent and predictive validity of the PS-proteins to serve as biomarkers of LN activity.
- Creator/Author:
- Tucker, Lori; Brunner, Hermine I.; Wiers, Kristina; Olson, Judyann; Suzuki, Michiko; Onel, Karen; Ying, Jun; Greis, Kenneth D.; Devarajan, Prasad; Haines, Kathleen; O'Neil, Kathleen M.; Silverman, Earl D.; Klein-Gitelman, Marisa S., and Brooks, Elizabeth B.
- Submitter:
- Kenneth Greis
- Date Uploaded:
- 03/03/2017
- Date Modified:
- 04/07/2017
- Date Created:
- 2009-02
- License:
- All rights reserved
-
- Type:
- Article
- Description/Abstract:
- OBJECTIVE: Damage to hair from UV exposure has been well reported in the literature and is known to be a highly complex process involving initiation via absorption of UV light followed by formation and propagation of reactive oxygen species (ROS). The objective of this work was to understand these mechanisms, explain the role of copper in accelerating the formation of ROS and identify strategies to reduce the hair damage caused by these reactive species. METHODS: The location of copper in hair was measured by Transmission electron microscopy–(TEM) X-ray energy dispersive spectroscopy (XEDS) and levels measured by ICP-OES. Protein changes were measured as total protein loss via the Lowry assay, and MALDI ToF was used to identify the biomarker protein fragments. TBARS assay was used to measure lipid peroxide formation. Sensory methods and dry combing friction were used to measure hair damage due to copper and UV exposure and to demonstrate the efficacy of N,N’ ethylenediamine disuccinic acid (EDDS) and histidine chelants to reduce this damage. RESULTS: In this work, a biomarker protein fragment formed during UV exposure is identified using mass spectrometry. This fragment originates from the calcium-binding protein S100A3. Also shown is the accelerated formation of this peptide fragment in hair containing low levels of copper absorbed from hair during washing with tap water containing copper ions. Transmission electron microscopy (TEM) X-ray energy dispersive spectroscopy (XEDS) studies indicate copper is located in the sulphur-poor endo-cuticle region, a region where the S100A3 protein is concentrated. A mechanism for formation of this peptide fragment is proposed in addition to the possible role of lipids in UV oxidation. A shampoo and conditioner containing chelants (EDDS in shampoo and histidine in conditioner) is shown to reduce copper uptake from tap water and reduce protein loss and formation of S100A3 protein fragment. In addition, the long-term consequences of UV oxidation and additional damage induced by copper are illustrated in a fourmonth wear study where hair was treated with a consumer relevant protocol of hair colouring treatments, UV exposure and regular shampoo and conditioning. CONCLUSIONS: The role of copper in accelerating UV damage to hair has been demonstrated as well as the ability of chelants such as EDDS and histidine in shampoo and conditioner products to reduce this damage.
- Creator/Author:
- McComb, D. W.; Marsh, J. M.; Sun, Y.; Chaudhary, T.; Flagler, M. J.; Coderch, L.; Mamak, M.; Greis, Kenneth D.; Rubio, L.; Williams, R. E. A., and Davis, M. G.
- Submitter:
- Kenneth Greis
- Date Uploaded:
- 03/03/2017
- Date Modified:
- 04/07/2017
- Date Created:
- 2015-04
- License:
- All rights reserved
-
- Type:
- Article
- Description/Abstract:
- In the pharmaceutical industry, there is a tremendous need for qualitative and quantitative analysis of target analytes such as peptides, proteins, drugs, metabolites, biomarkers, impurities, and degradation products in various mixtures including synthetic reactions, in vitro cultures, biological fluids, drug substances, finished products, and many others. To provide adequate specificity for analysis in these complex mixtures, multidimensional analytical techniques are required. Mass spectrometry plays a central role in many of these multidimensional approaches to mixture analysis because it provides an unparalleled combination of sensitivity and specificity that is useful for both molecular identification and quantitative applications. Recent innovations in mass spectrometry and industrial implementation of these advances have transformed many aspects of pharmaceutical research and development. Data that were previously unattainable, or were not collected due to exorbitant cost or time constraints, can now be obtained using mass spectrometry-based technologies. The impact of these innovations has been most dramatically felt in early stages of discovery, as more data are available to make critical decisions, such as selecting compounds for advancement to costly preclinical and clinical trials. New MS technologies have also accelerated the progression of drug candidates through development and toward regulatory approval. Here, five major categories of pharmaceutical applications of mass spectrometry are reviewed. They are new chemical entity characterization, biomacromolecule characterization, bioanalytical quantitation, metabolite identification, and impurity and degradation product identification. A brief historical perspective and evolution of technologies for each application area are presented. Those discussions are followed with a description of the current strategies for implementation of the tremendous capabilities of the state-of-the-art approaches, along with representative applications. In addition, emerging technologies for each application area are presented to indicate the future directions of instrumentation for mixture analysis in the pharmaceutical industry. (Int J Mass Spectrom 212 (2001) 135–196) © 2001 Elsevier Science B.V.
- Creator/Author:
- Harbol, Kevin L.; Greis, Kenneth D.; Hoke, Steven H.; Morand, Kenneth L.; Baker, Timothy R., and Dobson, Roy L. M.
- Submitter:
- Kenneth Greis
- Date Uploaded:
- 03/03/2017
- Date Modified:
- 04/07/2017
- Date Created:
- 2001-11
- License:
- All rights reserved
-
- Type:
- Article
- Description/Abstract:
- Candida albicans is a leading pathogen in infections of central venous catheters, which are frequently infused with heparin. Binding of C. albicans to medically relevant concentrations of soluble and plate-bound heparin was demonstrable by confocal microscopy and enzyme-linked immunosorbent assay (ELISA). A sequencebased search identified 34 C. albicans surface proteins containing ≥1 match to linear heparin-binding motifs. The virulence factor Int1 contained the most putative heparin-binding motifs (n = 5); peptides encompassing 2 of 5 motifs bound to heparin-Sepharose. Alanine substitution of lysine residues K805/K806 in 804QKKHQIHK811 (motif 1 of Int1) markedly attenuated biofilm formation in central venous catheters in rats, whereas alanine substitution of K1595/R1596 in 1593FKKRFFKL1600 (motif 4 of Int1) did not impair biofilm formation. Affinity-purified immunoglobulin G (IgG) recognizing motif 1 abolished biofilm formation in central venous catheters; preimmune IgG had no effect. After heparin treatment of C. albicans, soluble peptides from multiple C. albicans surface proteins were detected, such as Eno1, Pgk1, Tdh3, and Ssa1/2 but not Int1, suggesting that heparin changes candidal surface structures and may modify some antigens critical for immune recognition. These studies define a new mechanism of biofilm formation for C. albicans and a novel strategy for inhibiting catheter-associated biofilms.
- Creator/Author:
- Andes, David R.; Lu, Jason Long; Tan, Queenie K., G.; Hostetter, Margaret K.; Greis, Kenneth D.; Zhang, Minlu; Orsborn, Kris I.; Porollo, Alexey, and Green, Julianne V.
- Submitter:
- Kenneth Greis
- Date Uploaded:
- 03/03/2017
- Date Modified:
- 04/07/2017
- Date Created:
- 2013-07
- License:
- All rights reserved